Cisplatin assay

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August undefined, 2024

WebSep 23, 2016 · The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in ovarian cancer, providing an... WebApr 11, 2024 · The poster shows the final data from a phase 2 study evaluating the ability of the DRP ® companion diagnostic for cisplatin to prospectively identify patients with metastatic breast cancer that...

New combination chemotherapy of cisplatin with an …

WebMar 1, 2024 · Cisplatin is a platinum containing compound used for the treatment of different types of cancers. Quantitative determination of cisplatin has been carried out … WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 … portman lights gdynia

Frontiers Overcoming Limitations of Cisplatin Therapy by …

After observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more WebFeb 15, 2024 · We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer and incubated with anti-USP14 (ab70311; Abcam), anti-EGFR (ab52894; Abcam), or normal IgG (sc-2027; Santa Cruz ... optionalpha trading calender

Application Note - Agilent Technologies

WebAug 13, 2024 · Annexin V/propidium iodide (PI) apoptosis assay Cells were seeded onto 6-well plates at a density of 2.5 × 10 5 cells/well and assigned into four groups. In the single treatment group, cells were... WebCisplatin An anticancer agent WARNING: This product can expose you to chemicals including Cisplatin, which is [are] known to the State of California to cause cancer. For … optionalpha tradierWebAdjuvant cisplatin, 70 mg every three weeks, was given, for a total of 1680 mg. In February 1979, while he was receiving cisplatin, fever developed, accompanied by dark urine, a … optionalpha/login

"WebCisplatin is a platinum-containing compound used for the treatment of different types of cancer. The quantitative determination of cisplatin has been carried out using several … " - Cisplatin assay

Cisplatin assay

Cisplatin Injection: MedlinePlus Drug Information

WebAssay preparation— Dissolve about 100 mg of Cisplatin, accurately weighed, in dimethylformamide in a 100-mL volumetric flask, dilute with dimethylformamide to volume, and mix. Chromatographic system (see … WebOct 22, 2024 · Cisplatin is a commonly used anticancer drug, but nephrotoxicity is a dose-limiting adverse effect. Recent experimental and clinical observations have demonstrated that multiple injections of...

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WebJan 18, 2024 · Cisplatin was administered by intraperitoneal injection in mouse. DNA damage, excision repair, and gene expression were assayed by Damage-seq, XR-seq, and RNA-seq. b PCA reveals tissue-specific... Web(A,B) MTT assay. The cisplatin-resistant cells and their parental cells were exposed to different concentrations of cisplatin for 72 h and viability was related to untreated …

WebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved … WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose …

WebBased on my testing, the best way is to dissolve cisplatin in 0.9% NaCl, and this approach can keep cisplatin stable not only at room temperature but also at 37-41°C. The solubility of... WebNov 1, 2008 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its applications to ...

WebMar 1, 2024 · Cisplatin (cis-diamminedicholroplatinum II, Fig. 1) is a platinum complex bound to two chloride atoms and two ammonia molecules which makes it highly soluble …

WebMay 26, 2024 · Cisplatin is a well-known chemotherapeutic drug for solid tumors and leukemia with muscle toxicity, and long-term cisplatin medication can induce aberrant ROS accumulation in muscle cells and result in systemic muscle impairments ( Sakai et al., 2014; Conte et al., 2024 ). optionalpha apiWebIntroduction: Cisplatin has been indicated for several malignancies all over the world for many years. Increasing patient tolerance for high dose of chemotherapeutics and … optionataWebTherapeutic efficacy of cisplatin-based treatment of late stage urothelial carcinoma (UC) is limited by chemoresistance. To elucidate underlying mechanisms and to develop new … portman material handlingWebSep 18, 2024 · Cisplatin is a medication used to manage and treat solid tumors and hematologic malignancies. It is in the alkylating agent class of cytotoxic medications. This activity reviews the indications, action, and … optionaltableconstraintWebNov 1, 2005 · The assay was calibrated by addition of known amounts of cisplatin to drug-free plasma as described previously. Samples used for calibration contained cisplatin … portman lounge harvey normanWebElectron microscopywas used to observe ultrastructure changes in cell. Results: The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism. portman logistics roughamWebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsistencies. We showed that IC50 errors caused by the technical deficiencies of the MTT assay are large and not adjustable (range: 300-11,000%). portman marina lake hartwell sc