Greengate cloning

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August undefined, 2024

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GreenGate - A Novel, Versatile, and Efficient Cloning …

WebGreenGate Cloning Kit Description: GreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs. It is based on the Golden Gate … WebCloning is performed by pipetting in a single tube all plasmid donors, the recipient vector, a type IIS restriction enzyme and ligase, and incubating the mix in a thermal cycler. … the overlook at bison ridge

Golden Gate (24 Fragment) Assembly Protocol NEB

WebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp D-dummy = 2252-2297bp ... GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J ... WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N-terminal tag, coding sequence, C-terminal tag, plant terminator and plant resistance cassette), but at the same time allows assembling several expression cassettes in one … Weband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … the overlook at bear creek fredericksburg

GreenGate---a novel, versatile, and efficient cloning system for …

GreenGate cloning technology introduces enormous …

WebFor this study, plasmid constructs were generated via GreenGate cloning . The protein-coding regions of MsSND1, ... For ectopic expression of transcription factors, the respective GreenGate construct was transformed into Agrobacterium tumefaciens ASE (pSOUP +). Selected transgenic clones were incubated in liquid LB medium containing the ... WebGreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed Plasmids from Article ... the overlook at briney creekWebOct 12, 2024 · The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. Sequences for cysteine … the overlook apartments wauwatosa maintenance

"WebGreenGate Cloning System to create plant expression vectors containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - … " - Greengate cloning

Greengate cloning

WebGreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS … WebGreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors. All these make GreenGate versatile and applicable cloning system …

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WebGolden Gate cloning is one of the easiest cloning methods in terms of hands-on time, as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector(s) are placed in a … WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N …

Web57 rows · GreenGate Cloning System(Kit # 1000000036 ) Depositing Lab: Jan Lohmann. The 56 plasmid GreenGate cloning kit can be used to create plant expression vectors containing several cassettes and generate multi … WebGolden Gate cloning is one of the easiest cloning methods in terms of hands-on time, as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector (s) are placed in a …

WebJan 4, 2024 · promoter. Harnessing the flexibility of the GreenGate cloning system, we 57 produced a comprehensive set of GR-LhG4 driver lines targeting most tissues in the 58 . Arabidopsis. shoot and root with a strong focus on the indeterminate meristems. We show that, 59 when combined with effectors under control of the . pOp WebOct 15, 2024 · One cloning kit, termed GreenGate, was designed to facilitate rapid cloning of . constructs using commonly used elements of plant transformation vectors [16]. Assembly .

WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of …

WebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … shurflo rv water pumps backflow preventerWebNow, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target organism. the overlook at buffalo parkWebJul 19, 2024 · The driver lines were generated employing the fast and flexible GreenGate cloning system (Lampropoulos et al., 2013) but are compatible with any vector/transgenic line in which the expression of an effector is under the control of derivatives of the pOp promoter element (Moore et al., 1998). An important feature of our driver lines is the ... the overlook apartments wauwatosa wiWebMay 14, 2024 · The Golden Gate cloning method is an easy and straight forward procedure. Here, Lampropoulos et al. describe a variety of it designed for plant … shurflo service bulletin #1031WebDec 15, 2024 · A universal cloning method and repository for Ulva vectors . 102. An important prerequisite for a molecular toolkit is a universal and cost-effective cloning . 103. method that can be easily adopted by different laboratories. To this end, we generated a set of . 104. plasmids based on the GreenGate cloning system ((Lampropoulos et al., 2013 ... shurflo rv water pump filtersWebJan 20, 2024 · Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis … shurflo rv water pump 4008WebAug 9, 2015 · The development of DNA cloning technologies and the discovery of Agrobacterium sp. as a vehicle for delivery of recombinant DNA into the chromosomes of … shurflo saltwater washdown pump