Hcs lipidtox

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August undefined, 2024

WebExcess lipid accumulates in vesicles that displace the cytoplasm; large accumulations of lipids can disrupt cell constituents, and in severe cases, the cell may even burst. Steatosis is a common consequence of hepatocellular insult, which can be drug-induced or can be an outcome in pathophysiogical disease progression. WebNov 9, 2024 · HCS LipidTOX dyes were developed to indicate the accumulation of neutral or polar lipids and have been used for the analysis of PLD in macrophages [ 11 ]. …

Methods to Assess Lipid Accumulation in Cancer Cells

WebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. Description; Category: Stain; MW: N/A. Stock Concentration: 1000 X. Preparation; Solvent for Stock Solution: DMSO. Storage; Temperature-20°C; Precaution(s) Protected from light; WebOct 30, 2024 · However, the combination of HCS LipidTOX™ steatosis reagents provides a labeling strategy to perform high content imaging multiplexed assays to characterize phospolipidosis and steatosis, and to measure the effects on lipid metabolism that can be triggered by drugs or other compounds. malaysia beach resorts tripadvisor

invitrogen赛默飞试剂10597012/11668019/G661012/H34477

WebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically to amplify lipid accumulation in the observed puncta. We note that the most significant increases in DUNN index were … WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically ... malaysia beauty industry

IJMS Free Full-Text Different Sensitivity of Macrophages to

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … WebA collection of spectra representing the microscope Nikon Ti2 on FPbase. malaysia beauty productsWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … malaysia beach vacation spots

"WebHCS LipidTOX™ phospholipidosis detec-tion reagents can be easily detected with fluorescence microscopes or HCS readers equipped with standard filter sets (Figure … " - Hcs lipidtox

Hcs lipidtox

Neutral lipid alterations in Human Herpesvirus 8-infected …

WebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is … WebAug 20, 2024 · To better characterize a potential role of TMEM41B in regulating LDs, we analyzed H4 cells basally or upon treatment with BSA-conjugated oleic acid by probing with HCS LipidTox Green Neutral Lipid Stain, a probe with high affinity for neutral LDs.

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WebHCS LipidTOX Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to … WebOct 30, 2024 · HCS LipidTOX™ Phospholipidosis and Steatosis detection kits; The kit has following components (a) Red phospholipidosis detection reagent (1000× stock). (b) …

WebMar 28, 2015 · HCS LipidTOX™ Red Neutral Lipid stain has an extremely high affinity for neutral lipid droplets and is best imaged with filter sets appropriate for Alexa Fluor® 594 dye or Texas Red® dye (ex577-em609 nm). The XY coordinates were annotated in order to recognize the same microscopic field. Treble staining allowed us to evaluate possible ... WebHCS LipidTOX™ 中性脂质染料可用于成像法高内涵筛选（HCS）检测，可表征化合物对哺乳动物细胞系中脂质代谢的潜在毒副作用。 LipidTOX™ 系列检测试剂盒包括用于固定细胞的中性脂质染料和用于活细胞的磷脂染料，两者可在多重分析中组合使用。选择 HCS LipidTOX™ 染料骨髓来源的 hMSC 脂质囊泡，用 LipidTOX™ Green 中性脂质染料染 …

WebHCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be … HCS LipidTOX™ Green neutral lipid stain was developed to characterize the … HCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebSep 26, 2024 · For time-lapse imaging, live cells were treated with different agents and HCS LipidTOX red neutral lipid dye (Invitrogen, 1:200) was immediately added. ... LipidTOX staining confirmed the increase in lipid-droplet accumulation in these cells (Fig. 6E, F). Thus, BMP2 is a positive regulator of lipogenic differentiation in human IPF lung ...

WebJun 17, 2024 · Lipid droplets were separately analyzed by subtracting the HCS LipidTOX deep red stain from the HCS CellMask green stain to generate dark circular reliefs. These reliefs were automatically detected …

WebDec 7, 2024 · Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of … malaysia beauty trendWebApr 28, 2016 · 2μL ethidium homodimer (LIVE/DEAD Viability/Cytotoxicity Kit; Molecular Probes) and 1μL of HCS LipidTOX Deep Red (Molecular Probes) was mixed into each well containing placental explants. After 30mins, 100μL of 10μM BODIPY-C 12 solution was added to each well and mixed by trituration (total well volume 500μL, final concentration … malaysia beauty micro influencer instagramWebCitations & References The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid … malaysia beach wedding venueWebInvitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging. High affinity for neutral lipid droplets. Brand: Invitrogen™ H34475. Code : 50. Additional Details : … malaysia beauty storeWebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … malaysia beauty fairWebAug 8, 2013 · The assays were carried out with the HCS LipidTOX Phospholipidosis and Steatosis Detection Kit according to the manufacturer's instructions (Invitrogen). Xenograft Analysis. Nude mice (nu/nu, 6- to 8-week-old males) were injected subcutaneously with 1.5 × 10 5 A549 stable cells. The diameters of tumors were measured every 10 days. malaysia beauty influencerWebNov 9, 2024 · In a study using HCS LipidTOX Green phospholipidosis detection reagent for the identification of changes in rat and human macrophages after exposure to 0.1, 1, and 10 µM amiodarone, the following results were obtained after 24 h of exposure . Rat primary alveolar macrophages reacted differently from NR8383 and human U937 macrophages … malaysia bed frame