Ion s5tm xl platform
Web3 mei 2024 · Multiplexed, single-end sequencing was performed on an Ion S5TM XL platform (600 bp) in Tianjin Novogene Bioinformatic Technology Co., Ltd., China. Sequence data were uploaded to NCBI Sequence Read Archive under … WebrRNA gene and ITS1 gene sequenced on an Ion S5TM XL platform (Wang et al., 2024) by Novogene Genomics Institute (Beijing, China). These raw reads are banked in the NCBI Sequence Read Archive (SRA) database under the accession PRJNA633325. Funding: This research was supported by the National Natural Science Foundation of China (grant no. …
Ion s5tm xl platform
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WebThe library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientic) and sequenced on an Ion S5TM XL platform. Data Analysis Downstream data analysis, such as sequence quality control, chimera removal, and species annotation, was performed as previously described [3]. WebApplications Ion S5TM and Ion S5TM XL Systems • Simplest NGS workflow for targeted sequencing - <15mins to set up a sequencing run and <45mins total hands-on time from …
WebSamples from two different positions (sites 1 and 2) at three different depths were evaluated, totaling six solid waste samples; two samples from an impacted stream were also collected. 16S rRNA... Web15 jul. 2024 · DNA extraction and sequencing using the platform Ion S5 XL, SE600. The genomic DNAs of solid waste and sediment samples (suspended solids from Up and …
Web23 apr. 2024 · Then, the qualified amplicon libraries were sequenced on the Ion S5TM XL platform (Thermo Scientific) following the manufacturer’s protocol. The sequencing reads were processed using QIIME pipeline (v1.9.1) . Quality filtering were performed under specific conditions according to the Cutadapt (Martin, 2011) . Chimera ... WebPlatform: Ion Torrent S5 XL: Library: Library name Construction protocol Strategy Source ... Finally, the library was sequenced on an Ion S5TM XL platform and 400 bp single-end …
WebTotal DNA samples were extracted and sequenced on the Ion S5 XL platform. After cutting off the barcodes and primers and filtering low-quality reads and chimeras, a total of …
Web18 jun. 2024 · Sequencing libraries were generated using Ion Plus Fragment Library Kit 48 rxns (Thermo Fisher, Waltham, MA, USA) following manufacturer's recommendations and were assessed on [email protected] Fluorometer (Thermo Fisher, Waltham, MA, USA) and then sequenced on an Ion S5TM XL platform. 16S rDNA sequencing data were saved in the … cancer in abdomen icd 10WebThe Ion S5 XL System is simple to use with cartridge-based reagents and offers superior scalability and flexibility to support a broad range of high throughput sequencing applications, from microbial genomes and gene panels to exomes and transcriptomes, using our 5 Series chips. For use with equipment: Ion Chef™ System. Features cancer in a bottleWeb15 aug. 2024 · At last, the library was sequenced on an Ion S5TM XL platform and 400 bp/600 bp single- end reads were generated. Statistical analysis. The analysis of variance for multiple comparisons was performed in Prisma soft- ware (version 5). Statistical differences were evalu- ated by analysis of variance (ANOVA) and Dunnett-t pairwise … cancer immunotherapy clinic ucsfWeb24 aug. 2024 · the Ion S5TM XL platform and it generated one-sided readings of 400 p.m./600 p.m. The amplicon was sequenced on the Illumina two-way platform to acquire … cancer immunotherapy winter school 2023Web20 okt. 2024 · Finally, the library was sequenced on an Ion S5TM XL platform, and 400 bp/600 bp single-end reads were generated. 2.3. Data Analysis 2.3.1. Single-End Read … cancer in a dog\u0027s legWebIon S5TM XL platform. e metagenomic and amplicon sequence data are deposited in the China National Gen-eBank DataBase (CNGBdb) database under the accession ID: … cancer in amish populationWebQubit 2.0 Fluorometer. The amplificates were sequenced on an Ion S5TM XL (Thermofisher, Shanghai, China) platform was used. Samples were examined for … cancer in ancient times