P53 dynamics control cell fate
Web(F) p53 dynamics and cell fate were measured by live-cell microscopy. Apoptotic cells were identified visually, in the bright-field channel (right panel, hour 18) and p53 levels were … Web(D) Left: Myc dynamics in cells born before hour 20 of imaging were grouped by cell fate (determined within 4 days after initial drug treatment). Mean ± SEM. Right: mean Myc-mNG levels in the first 3 h of drug treatment. Boxes denote 75 th, 50 th, and 25 th percentiles. Two-sided t test. From left to right, n = 25, 19, and 16 cells.
P53 dynamics control cell fate
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Webto investigate how the dynamic response of MAPK and p53 may intersect in determining cell fate during the G2 checkpoint. We reveal the previously unnoticed pulsatile activation of MAPK signaling after DNA damage and show how it intersects with the cell-autonomous control of p53 dynamics. We find that WebFeb 9, 2024 · To determine whether the dynamical patterns in p53 levels were reflected in the expression of its target genes, we quantified the mRNA expression dynamics of three …
WebMay 16, 2011 · We constructed an integrative model of the p53 network composed of four modules: a DNA repair module, an ATM sensor, a p53-centered feedback control module, … WebFeb 18, 2024 · Cell-autonomous changes in p53 expression govern the duration and outcome of cell-cycle arrest at the G2 checkpoint for DNA damage. Here, we report that mitogen-activated protein kinase (MAPK) signaling integrates extracellular cues with p53 dynamics to determine cell fate at the G2 checkpoint.
Webp53 dynamics control cell fate. Science. 2012; 336: 1440-1444. Crossref; PubMed; Scopus (542) Google Scholar ... In contrast to the lower level of transcription of most genes in GFP control cells, most p53 target genes had elevated transcription 2.5–3.5 hr after NCS treatment compared with untreated cells, ... Web(D) Left: Myc dynamics in cells born before hour 20 of imaging were grouped by cell fate (determined within 4 days after initial drug treatment). Mean ± SEM. Right: mean Myc …
WebDec 6, 2024 · p53 dynamics regulate cell fate in response to DNA damage (Purvis et al , 2012; Paek et al , 2016; Yang et al , 2024 ). To determine whether cells could induce …
WebApr 27, 2024 · Puf-A, a nucleolar Puf domain protein, is required for ribosome biogenesis. A study of Puf-A in zebrafish has shown that Puf-A is highly expressed in primordial germ cells (PGCs) and participates in PGC development. However, it remains unclear how Puf-A governs PGC development in mammals. Here, we generated transgenic mice carrying … blackrock lifepath ret nWebJan 7, 2016 · Signaling dynamics are known to encode and decode cellular information that controls cellular responses (Purivs and Lahav, 2013). p53 dynamics can control cell fate decision in response to DSBs, and cells that experience p53 pulses recover from DNA damage, whereas cells that are exposed to sustained p53 signaling frequently undergo … garmin tread lxWebJun 1, 2012 · Cells transmit information through molecular signals that often show complex dynamical patterns. The dynamic behavior of the tumor suppressor p53 varies depending … garmin treadmill testWebJan 23, 2024 · The dynamics model of tumor suppressor p53 system is employed to investigate how PTEN protein mediates p53 dynamics after DNA damage and it indicates that the moderate p53-dependent PTEN synthesis rate is required for p53 to oscillate, while the higher p53-dependent PTEN synthesis rate is needed for the p53 bi-stable switch. 1 … garmin tread m-sWebApr 11, 2024 · As the variable p53 levels resulted from the distinct p53 dynamics played a key role in promoting alternative cell fate outcomes of cell cycle arrest and cell death, as we elucidated before [ 15 ], it is important to understand the quantitative and mechanistic basis of the differential dynamic outputs of p53 pathway, which we elaborated below. garmin tread m sWebInduction of p53 triggers multiple cellular programs ranging fromtransientresponses,suchasDNArepairand cell cycle arrest, to terminal fates such as … blackrock lifepath retWebApr 12, 2024 · Axin2-knockdown and control cells were mixed at a 1:10 ratio. Mixed cells are subjected to flow cytometry analysis at 1–3 days post mixing (dpm). ... indicating that … blackrock lifepath retirement 2025