Phosphinothricin n-acetyltransferase
WebNational Center for Biotechnology Information WebNov 21, 2016 · Abstract. Acetylation of small molecules is widespread in nature, and in some cases, cells use this process to detoxify harmful chemicals. Streptomyces species …
Phosphinothricin n-acetyltransferase
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WebMay 5, 2024 · The aminoglycoside 6'-N-acetyltransferase type Ib encoded by Tn1331 is evenly distributed within the cell's cytoplasm. Antimicrob Agents Chemother. 2003 …
WebBiologically, phosphinothricin is a structural mimic of γ-glutamyl phosphate and an inhibitor of glutamine synthetase. 24 Phosphinothricin is first phosphorylated by Gln synthetase, and then the phosphorylated intermediate inactivates the enzyme. 24,25 Gln synthetase inhibition results in accumulation of glutamate and ammonia, with the latter … WebJun 1, 2014 · Phosphinothricin Phosphinothricin-N-acetyltransferase Cold-adapted enzyme 1. Introduction Recently, much attention has been focused on the enzymatic modification of nonselective herbicides, owing to the potential application of the genes encoding such enzymes in agricultural biotechnology.
WebBioCrick BioTech 88# Keyuan road,Hi-Tech Zone Chengdu, Sichuan 610042, PRC Email: [email protected] Tel:86-28-8543-3893 WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring …
WebGmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance …
WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from Streptomyces viridochromogenes, are present in glufosinate-ammonium-tolerant plant varieties of various crops such as corn, cotton, rice, oilseed rape, and soybean. birch\\u0027s schoolwear port elizabethWebMar 20, 2006 · Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N -acetyltransferase to detoxify glufosinate. Glufosinate-tolerant crops have been commercialized using one of these two transgenes. Download to read the full … dallas remington songwriterWebNov 27, 2024 · Abstract Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops. birch\u0027s shoes in oakway center eugeneWebMay 26, 2024 · PAT protein overexpression and purification. PAT (phosphinothricin acetyltransferase) expression condition were varied to obtain high yield, and transformed E. coli was examined in various condition such as temperature, IPTG concentration and culturing time. IPTG concentration of 0, 0.5 and 1 mM concentration were used at 18 °C, … dallas remote workplaceWebPhosphinothricin acetyltransferase acetylates the free NH 2-group of glufosinate ammonium, thereby preventing accumulation of ammonia. Method of Genetic … birch\\u0027s shoe lacesWebL-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via Agrobacterium-mediated gene … dallas remote learningPhosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin birch\\u0027s supper club